The Electron Transport Chain and ATP Synthesis

These ATP molecules ultimately provide the energy for most of the cell's reactions.

Just as man-made power plants produce electrical energy by using the flow of wind, water, or steam to rotate a turbine, the synthase makes ATP by using proton flow from one side of the inner membrane to the other to rotate protein subunits.

If there is no proton gradient, synthase subunits stop rotating, and the cell can quickly become starved of energy and die.

Therefore, the protein complexes and small molecules that establish this gradient and maintain it play an essential role in the life of the cell.

At the heart of this system are four protein complexes, numbered one through four.

Complexes one, three, and four directly pump protons from the matrix into the intermembrane space.

Complex two does not directly pump protons, but it does promote proton pumping in complexes three and four.

Proton pumping requires energy, and the four protein complexes get this energy by transferring electrons through a series of coupled reactions.

This linked process of electron transport is why the four complexes are collectively referred to as the electron transport chain.

Let's focus on complex one.

A by-product of sugar metabolism called NADH deposits two high-energy electrons in complex one, where they are passed along a chain of redox centers.

Redox centers are clusters of atoms that have different affinities for electrons based on their unique atomic configurations.

Let's closely consider a pair of redox centers to reveal two reasons why an electron moves from the top redox center to the bottom.

First, the bottom redox center has higher affinity than the top one.

Second, the distance between these adjacent redox centers is ideal for an electron jump to occur, which explains why electrons typically don't bypass the bottom redox center.

A small amount of energy is released each time an electron is passed between redox centers.

Complex one harnesses this energy across all the redox centers and uses it to pump protons.

The last redox center in complex one donates two electrons to a coenzyme Q molecule.

Complex two is similar to complex one in two important ways.

First, high-energy electrons also enter complex two via a by-product of sugar metabolism, although here the molecule is FADH2.

Second, complex two also transfers electrons between several redox centers before donating them to coenzyme Q. One major difference, however, is that complex two does not use the energy liberated to pump protons.

Coenzyme Q molecules from complexes one and two donate their electrons to complex three.

One electron is recyclable and can re-enter complex three later, but the other passes through two redox centers before reaching cytochrome C. Cytochrome C carries the electron to complex four.

The electron transport chain ends in complex four, where a series of reactions involving four electrons converts a molecule of oxygen to two molecules of water.

The proton gradient is strengthened because four protons from the matrix are incorporated into water molecules, and another four are pumped into the intermembrane space.

In the absence of oxygen, the electron transfer comes to a halt, meaning that ATP synthesis also stops.

Indeed, the reason we breathe oxygen is so that it can serve as the final electron acceptor at the end of the electron transport chain.

In this animation, we have explored each protein complex in isolation, but in reality they are very densely packed.

Together, they effectively make the entire surface of the inner mitochondrial membrane a giant cellular power plant.